Translational_Unit
Part:BBa_K5078003:Design
Designed by: Callie Ross Group: iGEM24_UVU-Utah-2 (2024-09-28)
PSR_1 level 1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 895
Illegal PstI site found at 1382
Illegal PstI site found at 1591
Illegal PstI site found at 1761
Illegal PstI site found at 2238
Illegal PstI site found at 2673
Illegal PstI site found at 2841 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2407
Illegal NheI site found at 3163
Illegal PstI site found at 895
Illegal PstI site found at 1382
Illegal PstI site found at 1591
Illegal PstI site found at 1761
Illegal PstI site found at 2238
Illegal PstI site found at 2673
Illegal PstI site found at 2841
Illegal NotI site found at 3626
Illegal NotI site found at 3716 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4037
Illegal BamHI site found at 4414 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 895
Illegal PstI site found at 1382
Illegal PstI site found at 1591
Illegal PstI site found at 1761
Illegal PstI site found at 2238
Illegal PstI site found at 2673
Illegal PstI site found at 2841 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 895
Illegal PstI site found at 1382
Illegal PstI site found at 1591
Illegal PstI site found at 1761
Illegal PstI site found at 2238
Illegal PstI site found at 2673
Illegal PstI site found at 2841
Illegal NgoMIV site found at 1615
Illegal NgoMIV site found at 1983
Illegal NgoMIV site found at 2016
Illegal NgoMIV site found at 2085
Illegal NgoMIV site found at 2108
Illegal NgoMIV site found at 2127
Illegal NgoMIV site found at 2153
Illegal AgeI site found at 914
Illegal AgeI site found at 2001 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
What ends to create so the Psr1 gene would fit into our plasmid.
Source
We built this part using golden gate cloning.