Part:BBa_K5078003:Design

PSR_1 level 1

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 895
  Illegal PstI site found at 1382
  Illegal PstI site found at 1591
  Illegal PstI site found at 1761
  Illegal PstI site found at 2238
  Illegal PstI site found at 2673
  Illegal PstI site found at 2841
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2407
  Illegal NheI site found at 3163
  Illegal PstI site found at 895
  Illegal PstI site found at 1382
  Illegal PstI site found at 1591
  Illegal PstI site found at 1761
  Illegal PstI site found at 2238
  Illegal PstI site found at 2673
  Illegal PstI site found at 2841
  Illegal NotI site found at 3626
  Illegal NotI site found at 3716
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 4037
  Illegal BamHI site found at 4414
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 895
  Illegal PstI site found at 1382
  Illegal PstI site found at 1591
  Illegal PstI site found at 1761
  Illegal PstI site found at 2238
  Illegal PstI site found at 2673
  Illegal PstI site found at 2841
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 895
  Illegal PstI site found at 1382
  Illegal PstI site found at 1591
  Illegal PstI site found at 1761
  Illegal PstI site found at 2238
  Illegal PstI site found at 2673
  Illegal PstI site found at 2841
  Illegal NgoMIV site found at 1615
  Illegal NgoMIV site found at 1983
  Illegal NgoMIV site found at 2016
  Illegal NgoMIV site found at 2085
  Illegal NgoMIV site found at 2108
  Illegal NgoMIV site found at 2127
  Illegal NgoMIV site found at 2153
  Illegal AgeI site found at 914
  Illegal AgeI site found at 2001
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

What ends to create so the Psr1 gene would fit into our plasmid.

Source

We built this part using golden gate cloning.