Translational_Unit

Part:BBa_K5078003:Design

Designed by: Callie Ross   Group: iGEM24_UVU-Utah-2   (2024-09-28)


PSR_1 level 1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 895
    Illegal PstI site found at 1382
    Illegal PstI site found at 1591
    Illegal PstI site found at 1761
    Illegal PstI site found at 2238
    Illegal PstI site found at 2673
    Illegal PstI site found at 2841
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2407
    Illegal NheI site found at 3163
    Illegal PstI site found at 895
    Illegal PstI site found at 1382
    Illegal PstI site found at 1591
    Illegal PstI site found at 1761
    Illegal PstI site found at 2238
    Illegal PstI site found at 2673
    Illegal PstI site found at 2841
    Illegal NotI site found at 3626
    Illegal NotI site found at 3716
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4037
    Illegal BamHI site found at 4414
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 895
    Illegal PstI site found at 1382
    Illegal PstI site found at 1591
    Illegal PstI site found at 1761
    Illegal PstI site found at 2238
    Illegal PstI site found at 2673
    Illegal PstI site found at 2841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 895
    Illegal PstI site found at 1382
    Illegal PstI site found at 1591
    Illegal PstI site found at 1761
    Illegal PstI site found at 2238
    Illegal PstI site found at 2673
    Illegal PstI site found at 2841
    Illegal NgoMIV site found at 1615
    Illegal NgoMIV site found at 1983
    Illegal NgoMIV site found at 2016
    Illegal NgoMIV site found at 2085
    Illegal NgoMIV site found at 2108
    Illegal NgoMIV site found at 2127
    Illegal NgoMIV site found at 2153
    Illegal AgeI site found at 914
    Illegal AgeI site found at 2001
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

What ends to create so the Psr1 gene would fit into our plasmid.


Source

We built this part using golden gate cloning.

References